{"id":306,"date":"2026-09-14T07:30:00","date_gmt":"2026-09-13T23:30:00","guid":{"rendered":"https:\/\/www.ivd-stage.com\/?p=306"},"modified":"2026-09-13T20:29:03","modified_gmt":"2026-09-13T12:29:03","slug":"%e7%bb%b4d%e5%bf%ab%e6%a3%80%e5%8d%81%e5%b9%b4%e5%81%9a%e4%b8%8d%e5%87%86%ef%bc%8c%e8%a2%ab%e4%b8%80%e4%b8%aa%e8%bf%9e%e6%8e%a5%e8%87%82%e7%a0%b4%e8%a7%a3%e4%ba%86%ef%bc%81%e6%8a%97%e4%bd%93%e8%af%86","status":"publish","type":"post","link":"http:\/\/www.ivd-stage.com\/index.php\/2026\/09\/14\/%e7%bb%b4d%e5%bf%ab%e6%a3%80%e5%8d%81%e5%b9%b4%e5%81%9a%e4%b8%8d%e5%87%86%ef%bc%8c%e8%a2%ab%e4%b8%80%e4%b8%aa%e8%bf%9e%e6%8e%a5%e8%87%82%e7%a0%b4%e8%a7%a3%e4%ba%86%ef%bc%81%e6%8a%97%e4%bd%93%e8%af%86\/","title":{"rendered":"\u7ef4D\u5feb\u68c0\u5341\u5e74\u505a\u4e0d\u51c6\uff0c\u88ab\u4e00\u4e2a\u8fde\u63a5\u81c2\u7834\u89e3\u4e86\uff01\u6297\u4f53\u8bc6\u522b\u5bc6\u7801\u9996\u6b21\u62c6\u5f00"},"content":{"rendered":"<p><!-- IVD-STAGE 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8px;background:#f7f8fa;border-radius:8px;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u60f3\u8981\u80fd\u505a\u6210\u8bd5\u7eb8\u6761\u768425(OH)-D3\u4f18\u826f\u6297\u4f53\uff1f\u5c31\u5728\u6c5f\u5357\u5927\u5b66\uff01<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">\u8fd1\u65e5\uff0c\u4e00\u7bc7\u6c5f\u5357\u5927\u5b66\u7814\u7a76\u56e2\u961f\u7684\u7814\u7a76\u8bba\u6587\u53d1\u8868\u5728Analytical Chemistry\u4e0a\uff0c\u4f7f\u7528\u8ba1\u7b97\u673a\u8f85\u52a9\u534a\u6297\u539f\u8bbe\u8ba1\u548c\u5206\u5b50\u5bf9\u63a5\uff0c<strong>IC50=24.8ng\/mL<\/strong>\uff0c LFIA <strong>5\u5206\u949f\u51fa\u7ed3\u679c<\/strong>\uff0c\u4e0eLC-MS\/MS\u7ebf\u6027\u4e00\u81f4\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">![[Pasted image 20260913200339.png]]<\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u96be\u5728\u54ea<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">25(OH)-D3\u662f\u7ef4D\u8425\u517b\u8bc4\u4f30\u7684\u91d1\u6807\u51c6\u6807\u5fd7\u7269\uff0c\u4f46\u68c0\u6d4b\u4e00\u76f4\u6709\u4e09\u5927\u96be\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u758f\u6c34<\/strong>\u300225(OH)-D3\u6c34\u6eb6\u6027\u6781\u5dee\uff0c\u4e0e\u8840\u6e05\u91cc\u7ef4\u751f\u7d20D\u7ed3\u5408\u86cb\u767d\uff08VDBP\uff09\u7d27\u5bc6\u7ed3\u5408\u300295%\u4ee5\u4e0a\u768425(OH)-D3\u90fd\u4e0d\u662f&#8221;\u6e38\u79bb&#8221;\u7684\uff0c\u68c0\u6d4b\u524d\u9700\u5148\u89e3\u79bb\uff0c\u8fd9\u4e00\u6b65\u505a\u4e0d\u5230\u4f4d\uff0c\u514d\u75ab\u68c0\u6d4b\u53ea\u662f\u7a7a\u8c08\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u4e0d\u8010\u53d7\u5149\u548c\u70ed<\/strong>\u300225(OH)-D3\u5206\u5b50\u91cc\u6709\u5171\u8f6d\u4e09\u70ef\u7ed3\u6784\uff0c\u89c1\u5149\u5bb9\u6613\u53d1\u751f\u5f02\u6784\u3001\u53d7\u70ed\u4f1a\u53d1\u751f\u5206\u5b50\u91cd\u6392\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u7ed3\u6784\u76f8\u4f3c<\/strong>\u300225(OH)-D2\u548cD3\u53ea\u5dee\u4fa7\u94fe\u4e0a\u4e00\u4e2a\u7ec6\u8282\uff0c\u7acb\u4f53\u5316\u5b66\u548c\u5b98\u80fd\u56e2\u5206\u5e03\u51e0\u4e4e\u4e00\u6837\u3002<\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u56db\u4e2a\u534a\u6297\u539f<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">\u7814\u7a76\u5148\u8bbe\u8ba1\u4e86<strong>\u56db\u4e2a\u5019\u9009\u534a\u6297\u539f\uff08H1-H4\uff09<\/strong>\uff0c\u572825(OH)-D3\u9aa8\u67b6\u7684\u4e0d\u540c\u4f4d\u70b9\u63a5\u534a\u7425\u73c0\u9178\u916f\u6216\u5316\u5b66\u8fde\u63a5\u81c2\u3002\u5173\u952e\u64cd\u4f5c\u662f\u8fde\u63a5<strong>\u8f7d\u4f53\u86cb\u767d<\/strong>\uff0c\u628a\u5173\u952e\u8868\u4f4d\u5b8c\u5168\u7a7a\u51fa\u6765\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u4f7f\u7528\u5206\u5b50\u6a21\u62df\u6280\u672f\uff0c\u6700\u4f4e\u80fd\u91cf\u6784\u8c61\u53e0\u52a0\u663e\u793a\u56db\u4e2a\u534a\u6297\u539f\u7684\u753e\u4f53\u6838\u5fc3\u4e0e\u6bcd\u5206\u5b50\u9ad8\u5ea6\u91cd\u5408\uff0c<strong>\u8fde\u63a5\u81c2\u6ca1\u6709\u7834\u574f\u9aa8\u67b6\u51e0\u4f55<\/strong>\uff0c\u8868\u4f4d\u5b8c\u6574\u6027\u5148\u4fdd\u4f4f\u3002\u9759\u7535\u52bf\uff08ESP\uff09\u5206\u5e03\u4e0a\uff0cH1\u548cH2\u4e0e\u5929\u713625(OH)-D3\u6700\u63a5\u8fd1\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u514d\u75ab\u68cb\u76d8\u6ef4\u5b9a\u7b5b\u9009\u4e0b\u6765\uff0c<strong>H1-6K\u514d\u75ab+H1-BSA90\u5305\u88ab<\/strong>\u7684\u7ec4\u5408\u6291\u5236\u7387\u6700\u9ad8\uff08<strong>67%<\/strong>\uff09\uff0c\u540e\u9762\u5168\u90e8\u5355\u6297\u5f00\u53d1\u90fd\u57fa\u4e8e\u8fd9\u4e00\u5bf9\u3002<\/p>\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u56db\u4e2a\u6c28\u57fa\u9178<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">\u6700\u7ec8\uff0c \u79d1\u7814\u56e2\u961f\u5f97\u5230\u5355\u6297<strong>mAb-1F3<\/strong>\uff08IgG2a\/\u03ba\uff09\uff0cic-ELISA\u7684<strong>IC50=24.8ng\/mL<\/strong>\uff0c\u7ebf\u6027\u533a\u95f4\u4e3a<strong>8.79-69.95ng\/mL<\/strong>\u3002\u4ea4\u53c9\u53cd\u5e94\u8868\u663e\u793a\uff0c\u4e0e<strong>25(OH)-D2\u53ea\u67095%<\/strong>\u7684\u53cd\u5e94\u7387\uff0c\u4e0e\u89c6\u9ec4\u9187\u3001VE\u3001VK1\u3001VK3\u3001\u80c6\u56fa\u9187\u3001\u5b55\u916e\u3001\u777e\u916e\u3001\u96cc\u4e8c\u9187<strong>\u7b49\u5168\u90e8<1%<\/strong>\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u5206\u5b50\u5bf9\u63a5\u7814\u7a76\u8868\u660e\uff0c\u6297\u4f53CDR\u533a<strong>\u56db\u4e2a\u5173\u952e\u6b8b\u57fa\u2014\u2014PHE52\u3001PHE91\u3001TYR32\u3001ILE100<\/strong>\u6784\u6210\u534f\u540c\u7ed3\u5408\u7f51\u7edc\u3002\u4e09\u4e2a\u82ef\u4e19\/\u916a\u6c28\u9178\u6b8b\u57fa\u7528<strong>\u03c0-alkyl\u76f8\u4e92\u4f5c\u7528<\/strong>\u7a33\u4f4f\u758f\u6c34\u753e\u4f53\u9aa8\u67b6\uff0c<strong>ILE100\u4e0eC25-OH\u5f62\u62102.8\u00c5\u7684\u6c22\u952e<\/strong>\u3002<\/p>\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">LFIA<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">\u80f6\u4f53\u91d1\u7ade\u4e89\u6cd5LFIA\uff0c<strong>5\u5206\u949f<\/strong>\u51fa\u7ed3\u679c\uff0c\u4e09\u4e2a\u771f\u5b9e\u57fa\u8d28\uff08\u751f\u725b\u4e73\/\u81b3\u98df\u8865\u5145\u5242\/\u4eba\u8840\u6e05\uff09\uff0c\u4e0eLC-MS\/MS\u76f4\u63a5\u5bf9\u7167\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u7075\u654f\u5ea6<\/strong><\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u88f8\u773c\u68c0\u51fa\u9650\u8fbe2000ng\/mL\u3001cut-off\u4e3a 20000ng\/mL\uff0cLC-MS\/MS\u80fd\u8fbe\u5230\u52300.2ng\/mL\uff0c\u5dee<strong>\u4e24\u4e2a\u6570\u91cf\u7ea7<\/strong>\u3002\u9760\u56fe\u50cf\u5224\u8bfb\uff0c\u6700\u4f4e\u68c0\u51fa\u9650LoD\u8fbe\u5230 <strong>186.57ng\/mL<\/strong>\u3002\u56de\u6536\u7387 <strong>92-112.45%<\/strong>\u3001\u6279\u5185CV <strong>2.4-5%<\/strong>\u3001\u6279\u95f4 CV <strong>4.9-6.7%<\/strong>\u3002\u8bd5\u7eb8\u6761\u572830\/90\/180\u5929\u50a8\u5b58\u540e\uff0c\u68c0\u6d4b\u7ed3\u679c\u65e0\u660e\u663e\u53d8\u5316\u3002<\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u4f5c\u4e3aPOCT\uff0c\u8db3\u591f\u4e86\uff01<\/strong><\/p>\n<blockquote style=\"background:#f6f8fb;border-left:4px solid #1f3a5f;margin:14px 0;padding:10px 14px;color:#555;font-size:15px;\"><p>Chen J, Liu L, Guo L, Wu A, Xu C, Wu X. Elucidating Antibody\u2013Hapten Recognition for 25-Hydroxyvitamin D3 toward a Point-of-Need Immunoassay. *Analytical Chemistry*. 2026;98(35):26076\u201326088. DOI: 10.1021\/acs.analchem.6c04364<\/p><\/blockquote>\n<\/section>\n<p>  <!-- \u6587\u672b\u547c\u544a --><\/p>\n<p style=\"margin:28px 0 8px;color:#1f3a5f;font-weight:bold;font-size:17px;\">\n<p>  <!-- \u5206\u9694\u7ebf --><\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:24px 0;\">\n<p>  <!-- \u5f80\u671f\u70ed\u70b9\u6587\u7ae0\uff08\u6bcf\u671f\u6362\uff09 --><\/p>\n<section style=\"background:#f6f8fb;border-left:4px solid #1f3a5f;padding:14px 16px;margin:0 0 20px;border-radius:0 6px 6px 0;\">\n<p style=\"margin:0 0 10px;font-weight:bold;color:#1f3a5f;font-size:15px;\">\u5f80\u671f\u70ed\u70b9\u6587\u7ae0<\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247485377&#038;idx=1&#038;sn=4e8e20422bd83cc154d5484b0037f0b0\" style=\"color:#1f6fb8;text-decoration:none;\">\u5927\u5bb6\u597d\uff0c\u6211\u53eb\u9b4f\u5927\u6d77\u3002\uff08\u5168\u6587\u5b8c\u6574\u7248\uff09<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484400&#038;idx=1&#038;sn=6b384d73a26a9c622c745275a86f59fd\" style=\"color:#1f6fb8;text-decoration:none;\">\u94fe\u9709\u4eb2\u548c\u7d20\u5fae\u7403\u662f\u5982\u4f55\u5236\u5907\u7684\uff1f<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484362&#038;idx=1&#038;sn=a68382db7d3008165c534d93125633aa\" style=\"color:#1f6fb8;text-decoration:none;\">\u6297\u4f53\u6807\u8bb0\u7528\u751f\u7269\u7d20\uff0c\u4f60\u9009\u5bf9\u4e86\u5417\uff1f<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484503&#038;idx=1&#038;sn=69c1df88923335f7cdfa9fdcd84bd519\" style=\"color:#1f6fb8;text-decoration:none;\">\u7a7a\u767d\u9650\u3001\u68c0\u51fa\u9650\u3001\u5b9a\u91cf\u9650\uff0c\u4e00\u7bc7\u5c31\u8ba9\u4f60\u660e\u767d\uff01<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u60f3\u8981\u80fd\u505a\u6210\u8bd5\u7eb8\u6761\u768425(OH)-D3\u4f18\u826f\u6297\u4f53\uff1f\u5c31\u5728\u6c5f\u5357\u5927\u5b66\uff01 \u8fd1\u65e5\uff0c\u4e00\u7bc7\u6c5f\u5357\u5927\u5b66\u7814\u7a76\u56e2\u961f\u7684\u7814\u7a76\u8bba\u6587\u53d1\u8868\u5728Ana [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[7],"tags":[],"class_list":["post-306","post","type-post","status-publish","format-standard","hentry","category-7"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - 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