{"id":259,"date":"2026-08-25T07:30:00","date_gmt":"2026-08-24T23:30:00","guid":{"rendered":"https:\/\/www.ivd-stage.com\/?p=259"},"modified":"2026-08-23T21:36:07","modified_gmt":"2026-08-23T13:36:07","slug":"%e5%85%8d%e5%8a%a0%e7%83%ad%e5%85%8d%e9%80%86%e8%bd%ac%e5%bd%95%ef%bc%9a%e6%8c%87%e6%95%b0%e6%bb%9a%e7%8e%af%e6%89%a9%e5%a2%9e%e6%8a%8a%e7%97%85%e6%af%92rna%e6%a3%80%e6%b5%8b%e7%9b%b4%e6%8e%a5","status":"publish","type":"post","link":"http:\/\/www.ivd-stage.com\/index.php\/2026\/08\/25\/%e5%85%8d%e5%8a%a0%e7%83%ad%e5%85%8d%e9%80%86%e8%bd%ac%e5%bd%95%ef%bc%9a%e6%8c%87%e6%95%b0%e6%bb%9a%e7%8e%af%e6%89%a9%e5%a2%9e%e6%8a%8a%e7%97%85%e6%af%92rna%e6%a3%80%e6%b5%8b%e7%9b%b4%e6%8e%a5\/","title":{"rendered":"\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761"},"content":{"rendered":"<p><!-- IVD-STAGE \u516c\u4f17\u53f7\u6392\u7248\u6a21\u677f\uff08\u5185\u8054\u6837\u5f0f\uff0c\u517c\u5bb9\u5fae\u4fe1\uff09\n\u53d8\u91cf\u5360\u4f4d\u7b26\uff08\u63a8\u9001\u811a\u672c\u4f1a\u66ff\u6362\uff09\uff1a\n  \u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761        \u6587\u7ae0\u6807\u9898\n  [CONTENT]        \u6b63\u6587 HTML\uff08\u7531 markdown \u8f6c\u6362\u5f97\u5230\uff09\n           \u53d1\u5e03\u65e5\u671f 2026\u5e748\u670811\u65e5\n      \u6587\u672b\u7f72\u540d\/\u547c\u544a\uff08\u53ef\u9009\uff09\n  {{RELATED_1..4}} \u5f80\u671f\u63a8\u8350 \u6807\u9898|\u94fe\u63a5 \uff08\u6bcf\u671f\u6362\uff0c\u53ef\u5c11\u586b\uff09\n--><\/p>\n<div style=\"font-family:-apple-system,'PingFang SC','Microsoft YaHei',sans-serif;color:#2b2b2b;font-size:15px;line-height:2;letter-spacing:0.5px;\">\n<p>  <!-- \u6b63\u6587 --><\/p>\n<section style=\"margin:0;padding:16px 8px;background:#f7f8fa;border-radius:8px;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761<\/h2>\n<blockquote style=\"background:#f6f8fb;border-left:4px solid #1f3a5f;margin:14px 0;padding:10px 14px;color:#555;font-size:15px;\"><p>McMaster\u5927\u5b66\u56e2\u961f\u5728Angew. Chem. Int. Ed.\u53d1\u8868\u65b0\u4f5c\uff1aRNase I\u5207RNA\u5f15\u7269 + \u6307\u6570\u6eda\u73af\u6269\u589e\uff08E-RCA\uff09+ \u94fe\u7f6e\u6362\uff08TMSD\uff09\u4e09\u6bb5\u5f0f\u63a5\u529b\uff0c\u628a\u7ed3\u6784\u5316\u75c5\u6bd2RNA\u76f4\u63a5\u53d8\u6210\u8bd5\u7eb8\u6761\u4e0a\u7684\u8089\u773c\u53ef\u89c1\u4fe1\u53f7\u3002LOD\u4f4e\u81f3500 aM\uff0c\u553e\u6db245\u5206\u949f\u51fa\u7ed3\u679c\uff0c\u7075\u654f\u5ea695%\u2013100%\u3001\u7279\u5f02\u6027100%\u3002<\/p><\/blockquote>\n<p style=\"margin:14px 0;text-align:justify;\"><img decoding=\"async\" src=\"https:\/\/www.ivd-stage.com\/wp-content\/uploads\/2026\/08\/Pasted-image-20260823212823.png\" style=\"max-width:100%;border-radius:4px;margin:8px 0;\" alt=\"fig\"\/><\/p>\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u4e09\u4e2a\u6570\u5b57<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>500 aM<\/strong><\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u68c0\u6d4b\u9650\u3002aM \u7ea7\uff0c\u76f8\u5f53\u4e8e\u6bcf\u6beb\u5347\u51e0\u5341\u4e2a\u62f7\u8d1d\u7684\u91cf\u7ea7\u300230\u5206\u949f\u7b49\u6e29\u53cd\u5e94\uff0c\u4e0d\u9700\u8981\u4efb\u4f55\u70ed\u5faa\u73af\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>45 min<\/strong><\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u553e\u6db2\u53d6\u6837\u5230\u8bd5\u7eb8\u6761\u8bfb\u7ed3\u679c\u7684\u5168\u6d41\u7a0b\u65f6\u95f4\u4e3a45 min\uff0c\u65e0\u987b\u52a0\u70ed\uff0c\u5168\u7a0b\u5ba4\u6e29\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\"><strong>95%\u2013100% \/ 100%<\/strong><\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u5bf940\u4f8b\u4e34\u5e8a\u553e\u6db2\u6837\u672c\uff0820\u4f8b\u9633\u6027\uff0c20\u9634\u6027\uff09\u7684\u56db\u4e2a\u57fa\u56e0\u7ec4\u533a\u57df\u9a8c\u8bc1\uff0c\u7075\u654f\u5ea6\u8fbe\u523095%\u2013100%\uff0c\u7279\u5f02\u6027100%\uff0cAUC\u5168\u90e81.00\u3002<\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">RCA<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">\u6eda\u73af\u6269\u589e\uff08RCA\uff09\u672c\u6765\u662f\u6700\u9002\u5408\u5e38\u6e29\u7684\u90a3\u4e00\u4e2a\uff0c\u03a629\u805a\u5408\u9176\u5ba4\u6e29\u5c31\u80fd\u8dd1\uff0c\u4ea7\u7269\u662f\u957f\u5355\u94fe\u4e32\u8054\u4f53\u3002\u4f46\u5b83\u6709\u4e2a\u81f4\u547d\u4f24\uff0c\u4ea7\u7269\u592a\u957f\uff0c\u5728\u8bd5\u7eb8\u6761\u7684NC\u819c\u4e0a\u6cf3\u52a8\u6548\u679c\u4e0d\u4f73\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u8be5\u7814\u7a76\u56e2\u961f\u4f7f\u7528\u72ec\u7279\u7684\u8bbe\u8ba1\u89e3\u51b3\u4e86\u8fd9\u4e2a\u95ee\u9898\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">RNase I\u628a\u57fa\u56e0\u7ec4RNA\u968f\u673a\u5207\u788e\uff0c\u552f\u72ec\u4e0e\u73af\u72b6DNA\u6a21\u677f\u7ed3\u5408\u7684\u76ee\u6807\u533a\u6bb5\u88ab\u4fdd\u62a4\u4e0b\u6765\uff0c\u76f4\u63a5\u5145\u5f53\u7b2c\u4e00\u4ee3\u5f15\u7269P1\uff0cRNA\u4e8c\u7ea7\u7ed3\u6784\u95ee\u9898\u4e5f\u987a\u624b\u89e3\u51b3\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">\u52a0\u4e00\u676130 nt\u7684\u7b2c\u4e8c\u4ee3\u5f15\u7269P2\uff0c\u7ed3\u5408\u5230RCA\u957f\u4ea7\u7269\u4e0a\u88ab\u03a629\u5ef6\u4f38\u3001\u7f6e\u6362\u51fa83 nt\u7684\u91cd\u590d\u5355\u5143P2EP\u3002P2EP\u518d\u54ac\u4f4f\u81ea\u5e268\u201310 bp\u81ea\u4e92\u8865\u533a\u7684\u73af\u72b6\u6a21\u677f\uff0c\u88ab\u03a629\u76843&#8217;\u20135&#8217;\u5916\u5207\u6d3b\u6027\u4fee\u63893&#8217;\u60ac\u5782\uff0c\u53d8\u6210\u7b2c\u4e09\u4ee3\u5f15\u7269P3\uff0c\u8fd9\u6837\uff0cP3\u548cP1\u4e00\u6837\u80fd\u542f\u52a8\u65b0\u4e00\u8f6eRCA\uff0c\u7ebf\u6027RCA\u5c31\u6b64\u53d8\u6307\u6570\u3002<\/p>\n<p style=\"margin:14px 0;text-align:justify;\">toehold\u4ecb\u5bfc\u7684\u94fe\u7f6e\u6362\u53cd\u5e94\u628aRCA\u5927\u4ea7\u7269\u53d8\u6210\u77ed\u7684\u6865\u63a5DNA\uff0c\u91ca\u653e\u51fa\u7684B-DNA\u5728\u8bd5\u7eb8\u6761\u68c0\u6d4b\u7ebf\u663e\u8272\u3002\u5927\u5206\u5b50\u8fc7\u4e0d\u4e86\u819c\u7684\u95ee\u9898\u88ab\u6210\u529f\u7ed5\u5f00\uff0c\u4e0d\u7528\u9176\u5207\u3001\u4e0d\u7528\u6807\u8bb0\u3002<img decoding=\"async\" src=\"https:\/\/www.ivd-stage.com\/wp-content\/uploads\/2026\/08\/Pasted-image-20260823212917.png\" style=\"max-width:100%;border-radius:4px;margin:8px 0;\" alt=\"fig\"\/><\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<h2 style=\"color:#1f3a5f;font-size:16px;font-weight:bold;margin:20px 0 10px;\">\u4e34\u5e8a\u8868\u73b0<\/h2>\n<p style=\"margin:14px 0;text-align:justify;\">40\u4f8b\u7ecfRT-qPCR\u68c0\u6d4b\u5df2\u786e\u8ba4\u5206\u7c7b\u7684\u553e\u6db2\u6837\u672c\uff0c\u8986\u76d6\u56db\u79cd\u75c5\u6bd2RNA\u533a\u57df\uff08NSP12\u3001NSP13\u3001NSP14\u3001NSP8\uff09\uff0c\u4e09\u4e2a\u7cfb\u7edf\u7075\u654f\u5ea695%\u2013100%\u3001\u7279\u5f02\u6027100%\uff0cROC\u66f2\u7ebfAUC=1.00\u3002\u800c\u5e02\u552eBTNX\u65b0\u51a0\u6297\u539f\u68c0\u6d4b\u8bd5\u5242\u6d4b\u540c\u4e00\u6279\u672a\u5904\u7406\u553e\u6db2\uff0c10\u4e2a\u9633\u6027\u91cc\u53ea\u6293\u52302\u4e2a\u3002<\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:22px 0;\">\n<p style=\"margin:14px 0;text-align:justify;\"><strong>\u4e0d\u52a0\u70ed\u3001\u4e0d\u63d0\u53d6\u3001\u4e0d\u9006\u8f6c\u5f55\u3001\u4e0d\u914dPCR\u4eea\uff0c\u8bd5\u7eb8\u6761\u8089\u773c\u8bfb\u6570\u3002<\/strong><\/p>\n<blockquote style=\"background:#f6f8fb;border-left:4px solid #1f3a5f;margin:14px 0;padding:10px 14px;color:#555;font-size:15px;\"><p>Amal Mathai, Monsur Ali, Yingfu Li, John D. Brennan. Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point-of-Care Detection of Viral RNA. *Angewandte Chemie International Edition*. 2026; e3766984. DOI: 10.1002\/anie.3766984<\/p><\/blockquote>\n<\/section>\n<p>  <!-- \u6587\u672b\u547c\u544a --><\/p>\n<p style=\"margin:28px 0 8px;color:#1f3a5f;font-weight:bold;font-size:17px;\">\n<p>  <!-- \u5206\u9694\u7ebf --><\/p>\n<p style=\"border-top:1px solid #e3e8ef;margin:24px 0;\">\n<p>  <!-- \u5f80\u671f\u70ed\u70b9\u6587\u7ae0\uff08\u6bcf\u671f\u6362\uff09 --><\/p>\n<section style=\"background:#f6f8fb;border-left:4px solid #1f3a5f;padding:14px 16px;margin:0 0 20px;border-radius:0 6px 6px 0;\">\n<p style=\"margin:0 0 10px;font-weight:bold;color:#1f3a5f;font-size:15px;\">\u5f80\u671f\u70ed\u70b9\u6587\u7ae0<\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247485377&#038;idx=1&#038;sn=4e8e20422bd83cc154d5484b0037f0b0\" style=\"color:#1f6fb8;text-decoration:none;\">\u5927\u5bb6\u597d\uff0c\u6211\u53eb\u9b4f\u5927\u6d77\u3002\uff08\u5168\u6587\u5b8c\u6574\u7248\uff09<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484400&#038;idx=1&#038;sn=6b384d73a26a9c622c745275a86f59fd\" style=\"color:#1f6fb8;text-decoration:none;\">\u94fe\u9709\u4eb2\u548c\u7d20\u5fae\u7403\u662f\u5982\u4f55\u5236\u5907\u7684\uff1f<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484362&#038;idx=1&#038;sn=a68382db7d3008165c534d93125633aa\" style=\"color:#1f6fb8;text-decoration:none;\">\u6297\u4f53\u6807\u8bb0\u7528\u751f\u7269\u7d20\uff0c\u4f60\u9009\u5bf9\u4e86\u5417\uff1f<\/a><\/p>\n<p style=\"margin:6px 0;font-size:14px;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5NjIzNDU4NQ==&#038;mid=2247484503&#038;idx=1&#038;sn=69c1df88923335f7cdfa9fdcd84bd519\" style=\"color:#1f6fb8;text-decoration:none;\">\u7a7a\u767d\u9650\u3001\u68c0\u51fa\u9650\u3001\u5b9a\u91cf\u9650\uff0c\u4e00\u7bc7\u5c31\u8ba9\u4f60\u660e\u767d\uff01<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761 McMaster\u5927\u5b66\u56e2\u961f\u5728Angew. Chem. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[7],"tags":[],"class_list":["post-259","post","type-post","status-publish","format-standard","hentry","category-7"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761 - IVD-STAGE<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.ivd-stage.com\/index.php\/2026\/08\/25\/\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2rna\u68c0\u6d4b\u76f4\u63a5\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761 - IVD-STAGE\" \/>\n<meta property=\"og:description\" content=\"\u514d\u52a0\u70ed\u514d\u9006\u8f6c\u5f55\uff1a\u6307\u6570\u6eda\u73af\u6269\u589e\u628a\u75c5\u6bd2RNA\u68c0\u6d4b\u76f4\u63a5\u642c\u4e0a\u8bd5\u7eb8\u6761 McMaster\u5927\u5b66\u56e2\u961f\u5728Angew. 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