{"id":235,"date":"2026-08-17T08:00:00","date_gmt":"2026-08-17T00:00:00","guid":{"rendered":"https:\/\/www.ivd-stage.com\/?p=235"},"modified":"2026-08-12T18:40:13","modified_gmt":"2026-08-12T10:40:13","slug":"40%e5%80%8d%e4%ba%b2%e5%92%8c%e5%8a%9b%ef%bc%81%e5%85%94%e5%8d%95%e6%8a%97%e6%8a%8a%e9%bc%a0%e5%8d%95%e6%8a%97%e6%8c%89%e5%9c%a8%e5%9c%b0%e4%b8%8a%e6%91%a9%e6%93%a6%ef%bc%81","status":"publish","type":"post","link":"http:\/\/www.ivd-stage.com\/index.php\/2026\/08\/17\/40%e5%80%8d%e4%ba%b2%e5%92%8c%e5%8a%9b%ef%bc%81%e5%85%94%e5%8d%95%e6%8a%97%e6%8a%8a%e9%bc%a0%e5%8d%95%e6%8a%97%e6%8c%89%e5%9c%a8%e5%9c%b0%e4%b8%8a%e6%91%a9%e6%93%a6%ef%bc%81\/","title":{"rendered":"40\u500d\u4eb2\u548c\u529b\uff01\u5154\u5355\u6297\u628a\u9f20\u5355\u6297\u6309\u5728\u5730\u4e0a\u6469\u64e6\uff01"},"content":{"rendered":"

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40\u500d\u4eb2\u548c\u529b\uff01\u5154\u5355\u6297\u628a\u9f20\u5355\u6297\u6309\u5728\u5730\u4e0a\u6469\u64e6\uff01<\/h2>\n

\u540c\u4e00\u9776\u70b9\uff0c40 \u500d\u4eb2\u548c\u529b\u78be\u538b\uff0c\u53ea\u662f\u7528\u4e86\u5154\u5355\u6297\u3002<\/strong><\/p>\n

\u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u738b\u6218\u8f89\u7814\u7a76\u56e2\u961f\u5728 Analytical Chemistry \u53d1\u8868\u5c01\u9762\u6587\u7ae0\uff0c\u4f7f\u7528\u5355\u7ec6\u80de\u5206\u9009\u6280\u672f\uff0c\u4ece\u5154\u813e\u810f\u91cc\u9009\u51fa 5 \u682a\u6297\u6c1f\u82ef\u5c3c\u8003\uff08FF\uff09\u53ca\u5176\u4ee3\u8c22\u7269\u6c1f\u82ef\u5c3c\u8003\u80fa\uff08FFA\uff09\u7684\u5154\u5355\u6297\uff0c\u5176\u4e2d RmAb-A4# \u5bf9 FF\u3001FFA \u7684 IC50 \u5206\u522b\u53ea\u6709 0.07 ng\/mL \u548c 0.16 ng\/mL<\/strong>\uff0c\u6bd4\u4e4b\u524d\u6700\u597d\u7684\u9f20\u5355\u6297 4F8 \u9ad8\u51fa 40 \u500d\u548c 20 \u500d<\/strong>\uff0c\u6bd4\u6587\u732e\u62a5\u9053\u8fc7\u7684\u6240\u6709\u6297\u4f53\uff08\u542b\u5154\u591a\u6297\uff09\u9ad8 2-463 \u500d\u3002<\/p>\n

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\u600e\u4e48\u7b5b\u51fa\u6765\u7684\uff1f<\/h2>\n

\u56e2\u961f\u7528\u7684 CSMN \u5e73\u53f0\uff0c\u4f7f\u752840 \u4e07\u4e2a\u7eb3\u7c73\u5b54\uff0c\u628a\u5154\u813e\u6dcb\u5df4\u7ec6\u80de\u505a\u5355\u7ec6\u80de\u60ac\u6d6e\u540e\uff0c\u4f7f\u7406\u8bba\u4e0a\u6bcf\u4e2a\u7eb3\u7c73\u5b54\u4ec5\u6709\u4e00\u4e2a\u7ec6\u80de\uff0c\u4f7f\u7528Cy3 \u8367\u5149\u5b9e\u65f6\u8ffd\u8e2a\u6297\u4f53\u7684\u5206\u6ccc\u60c5\u51b5\uff0c\u7ecf\u8fc7\u7ea68 \u5c0f\u65f6\u8fde\u7eed\u6210\u50cf\uff0c\u9633\u6027\u5b54\u4ece\u7b2c 2 \u5c0f\u65f6\u5f00\u59cb\u51fa\u73b0\u8367\u5149\u4fe1\u53f7<\/strong>\u3002\u7ed3\u679c\u663e\u793a\uff0c\u4e24\u53ea\u5154\u5b50\u5206\u522b\u53ea\u6709 33 \u548c 21 \u4e2a\u5b54<\/strong>\u51fa\u73b0\u8367\u5149\u4fe1\u53f7\u3002<\/p>\n

\u901a\u8fc7\u5bf9\u9633\u6027\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u8fdb\u884c\u91cd\u7ec4\u8868\u8fbe\uff0c\u5f97\u5230 5 \u682a\u529f\u80fd\u6027\u5154\u5355\u6297\uff0c\u6210\u529f\u7387 16.7%<\/strong>\uff0c\u8f83\u4f20\u7edf\u6742\u4ea4\u7624\u5e73\u53f0\u5154\u5355\u6297\u7684\u9633\u6027\u7387 1.9% \u8fd8\u662f\u8981\u9ad8\u4e0d\u5c11\u3002<\/p>\n

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\u901a\u8fc7\u5206\u5b50\u5bf9\u63a5\u548c\u52a8\u529b\u5b66\u6a21\u62df\u5bf9\u5176\u5206\u5b50\u673a\u5236\u7ed9\u4e86\u89e3\u91ca\u3002\u5154\u5355\u6297RmAb-A4# \u7684 Fab \u4e0a\u6709 6 \u4e2a\u4e8c\u786b\u952e<\/strong>\uff08\u5c0f\u9f20 4 \u4e2a\uff09\uff0c\u5bf9 FF\/FFA \u5f62\u6210 3-4 \u4e2a\u6c22\u952e+\u5364\u952e+\u03c0-\u03c0 \u5806\u79ef\uff1b\u7ed3\u5408\u81ea\u7531\u80fd \u0394G \u221222.01\/\u221218.81 kcal\/mol\uff0c\u800c\u5c0f\u9f20\u5355\u6297\u53ea\u6709 \u22122.17\/\u22128.75\uff0c\u4e24\u8005\u76f8\u5dee\u4e00\u4e2a\u6570\u91cf\u7ea7\u3002<\/p>\n

\u6297\u4f53\u7684\u7a33\u5b9a\u6027\u8868\u73b0\u4e5f\u975e\u5e38\u4f18\u79c0\uff0c\u572818-5000 mM<\/strong>\u7684NaCl\u8303\u56f4\u5185\uff0c\u5176 IC50 \u7a33\u5728 0.07-0.19 ng\/mL\uff1b\u5728pH 4-9 \u7684\u73af\u5883\u4e2d\uff0c\u4f9d\u65e7\u80fd\u4fdd\u6301\u6d3b\u6027\uff1b\u7532\u9187\u6d53\u5ea6 <15%\u3001\u4e59\u8148 \u6d53\u5ea6<5% \u7684\u6761\u4ef6\u4e0b\uff0c\u4ecd\u4e0d\u53d7\u5e72\u6270\u3002<\/p>\n

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\u56e2\u961f\u57fa\u4e8e RmAb-A4# \u7684\u91d1\u6807\u8bd5\u7eb8\u6761\u8fdb\u884c\u6027\u80fd\u8bc4\u4f30\uff0c\u5728\u725b\u5976<\/strong>\u57fa\u8d28\u4e0b\uff0c\u65e0\u524d\u5904\u7406\uff0cFF\/FFA \u76ee\u89c6\u68c0\u51fa\u9650\u5206\u522b\u4e3a 0.5\/0.7 \u03bcg\/L\uff0c\u534a\u5b9a\u91cf LoD\u4e3a 0.22\/0.17 \u03bcg\/L<\/strong>\uff1b\u5728\u9e21\u86cb<\/strong>\u57fa\u8d28\uff0c\u505a 3 \u500d\u7a00\u91ca\u4e0b\uff0c\u534a\u5b9a\u91cfLoD\u5206\u522b\u8fbe\u5230 0.74\/0.78 \u03bcg\/kg\u3002\u56de\u6536\u7387\u8fbe\u5230 80-110%\uff0c\u68c0\u6d4b\u5168\u7a0b\u8017\u65f6\u4e0d\u5230 10 \u5206\u949f\u3002<\/p>\n

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40\u500d\u4eb2\u548c\u529b\uff0c\u5fc3\u52a8\u4e0d\u5982\u884c\u52a8\uff01<\/h2>\n

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\u6587\u732e\u4fe1\u606f<\/strong>\uff1aLi P, Pan Y, Li Y, et al. Single-Cell-Derived Rabbit mAb-Based Immunoassay for Simultaneous Detection of Florfenicol and Florfenicol Amine. *Anal. Chem.* 2026, 98(29): 21280-21291. DOI: 10.1021\/acs.analchem.6c01192<\/a><\/p>\n<\/section>\n

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\u5f80\u671f\u70ed\u70b9\u6587\u7ae0<\/p>\n

\u5927\u5bb6\u597d\uff0c\u6211\u53eb\u9b4f\u5927\u6d77\u3002\uff08\u5168\u6587\u5b8c\u6574\u7248\uff09<\/a><\/p>\n

\u94fe\u9709\u4eb2\u548c\u7d20\u5fae\u7403\u662f\u5982\u4f55\u5236\u5907\u7684\uff1f<\/a><\/p>\n

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\u7a7a\u767d\u9650\u3001\u68c0\u51fa\u9650\u3001\u5b9a\u91cf\u9650\uff0c\u4e00\u7bc7\u5c31\u8ba9\u4f60\u660e\u767d\uff01<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

40\u500d\u4eb2\u548c\u529b\uff01\u5154\u5355\u6297\u628a\u9f20\u5355\u6297\u6309\u5728\u5730\u4e0a\u6469\u64e6\uff01 \u540c\u4e00\u9776\u70b9\uff0c40 \u500d\u4eb2\u548c\u529b\u78be\u538b\uff0c\u53ea\u662f\u7528\u4e86\u5154\u5355\u6297\u3002 \u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u738b\u6218\u8f89 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