{"id":202,"date":"2026-08-06T09:00:00","date_gmt":"2026-08-06T01:00:00","guid":{"rendered":"https:\/\/www.ivd-stage.com\/?p=202"},"modified":"2026-08-02T20:44:57","modified_gmt":"2026-08-02T12:44:57","slug":"4-am%ef%bc%8110%e5%88%86%e9%92%9f%e8%82%89%e7%9c%bc%e5%87%ba%e7%bb%93%e6%9e%9c%ef%bc%81%e8%82%a0%e7%99%8cmirna%e8%af%95%e7%ba%b8%e6%9d%a1%ef%bc%8c%e7%81%b5%e6%95%8f%e5%ba%a6%e6%af%94%e8%80%81%e6%96%b9","status":"publish","type":"post","link":"http:\/\/www.ivd-stage.com\/index.php\/2026\/08\/06\/4-am%ef%bc%8110%e5%88%86%e9%92%9f%e8%82%89%e7%9c%bc%e5%87%ba%e7%bb%93%e6%9e%9c%ef%bc%81%e8%82%a0%e7%99%8cmirna%e8%af%95%e7%ba%b8%e6%9d%a1%ef%bc%8c%e7%81%b5%e6%95%8f%e5%ba%a6%e6%af%94%e8%80%81%e6%96%b9\/","title":{"rendered":"4 aM\uff0110\u5206\u949f\u8089\u773c\u51fa\u7ed3\u679c\uff01\u80a0\u764cmiRNA\u8bd5\u7eb8\u6761\uff0c\u7075\u654f\u5ea6\u6bd4\u8001\u65b9\u6cd5\u9ad8120\u500d"},"content":{"rendered":"<div style=\"font-family:-apple-system,'PingFang SC','Microsoft YaHei',sans-serif;color:#2b2b2b;font-size:16px;line-height:1.8;max-width:720px;margin:0 auto;\">\n<p style=\"margin:12px 0;line-height:1.9;\"><strong>10\u5206\u949f\uff0cLOD 4\u00d710\u207b\u00b3 fM\uff08=4 aM\uff09\uff0c\u65e0\u9700\u4eea\u5668\uff0c\u8089\u773c\u8bfb\u6570<\/strong>\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">4 aM\u662f\u4e2a\u4ec0\u4e48\u6982\u5ff5\uff1f<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u53f0\u6e7e\u4e2d\u539f\u5927\u5b66\u7684\u7814\u7a76\u56e2\u961f\u628a\u5229\u7528&#8221;\u6307\u6570\u6eda\u73af\u6269\u589e+\u4fa7\u5411\u5c42\u6790&#8221;\uff08ERCA-LFA\uff09\uff0c\u5b9e\u73b06\u5206\u949f\u6269\u589e\u300110\u5206\u949f\u5168\u6d41\u7a0b\u3001\u8089\u773c\u76f4\u63a5\u8bfb\u7ed3\u679c\uff0c\u68c0\u6d4b\u4e0b\u9650\u8fbe4 aM\uff0c\u6bd4\u73b0\u6709miR-141\u68c0\u6d4b\u65b9\u6cd5\u7075\u654f120\u500d\u4ee5\u4e0a\u3002\u8fd9\u9879\u7814\u7a76\u53d1\u8868\u5728Talanta\u4e0a\uff0c<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.ivd-stage.com\/wp-content\/uploads\/2026\/08\/teng_talanta_title.png\" alt=\"Talanta\u8bba\u6587\u6807\u9898\u9875\" style=\"max-width:100%;height:auto;border-radius:6px;margin:14px 0;\" \/><\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<h2 style=\"color:#1f3a5f;margin:24px 0 12px;\">\u4e3a\u4ec0\u4e48miRNA\u8bd5\u7eb8\u6761\u96be\u505a\uff1f<\/h2>\n<p style=\"margin:12px 0;line-height:1.9;\">\u7ed3\u76f4\u80a0\u764c\u662f\u5168\u7403\u7b2c\u4e09\u5927\u764c\u79cd\uff0c2020\u5e74\u65b0\u589e\u7ea6190\u4e07\u4f8b\uff0c\u6b7b\u4ea1\u7ea693.5\u4e07\u4f8b\uff0c\u6b7b\u4ea1\u7387\u6392\u7b2c\u4e8c\uff0c\u4ec5\u6b21\u4e8e\u80ba\u764c\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u73b0\u6709\u7b5b\u67e5\u624b\u6bb5\u5404\u6709\u7f3a\u70b9\uff1a\u7caa\u4fbf\u9690\u8840\uff08iFOBT\uff09\u5047\u9633\u6027\u591a\uff0c\u75d4\u75ae\u3001\u6e83\u75a1\u90fd\u80fd\u5e72\u6270\uff1bCEA\u7b49\u80bf\u7624\u6807\u5fd7\u7269\u7279\u5f02\u6027\u6709\u9650\uff0c\u522b\u7684\u764c\u548c\u708e\u75c7\u4e5f\u5347\u9ad8\uff1b\u80a0\u955c+\u6d3b\u68c0\u662f\u91d1\u6807\u51c6\uff0c\u4f46\u6709\u521b\u3001\u6709\u5e76\u53d1\u75c7\u98ce\u9669\uff0c\u60a3\u8005\u4f9d\u4ece\u6027\u5dee\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">miR-141\u662fmiR-200\u5bb6\u65cf\u6210\u5458\uff0c\u5728\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u4e2d\u663e\u8457\u4e0a\u8c03\uff0c\u4e0e\u80bf\u7624\u8fdb\u5c55\u548c\u4e0d\u826f\u9884\u540e\u76f8\u5173\uff0c\u800c\u4e14\u5c3f\u6db2\u91cc\u5c31\u80fd\u68c0\u51fa\u5fae\u91cf\uff0c\u662f\u65e0\u521b\u65e9\u7b5b\u7684\u7406\u60f3\u9776\u6807\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u4f46miRNA\u5728\u4f53\u6db2\u91cc\u6d53\u5ea6\u6781\u4f4e\uff0c\u666e\u901a\u8bd5\u7eb8\u6761\u7684\u6297\u4f53\u8bc6\u522b\u6839\u672c\u591f\u4e0d\u7740\uff0c\u5047\u9634\u6027\u662f\u5e38\u6001\u3002<\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<h2 style=\"color:#1f3a5f;margin:24px 0 12px;\">\u628aRCA\u4ece&#8221;\u7ebf\u6027&#8221;\u6539\u6210&#8221;\u6307\u6570&#8221;<\/h2>\n<p style=\"margin:12px 0;line-height:1.9;\">\u6eda\u73af\u6269\u589e\uff08RCA\uff09\u662f\u7b49\u6e29\u6269\u589e\u7684\u8001\u6280\u672f\uff1aphi29\u805a\u5408\u9176\u7ed5\u7740\u73af\u72b6\u6a21\u677f\u8f6c\u5708\uff0c30\u00b0C\u6052\u6e29\u4e0b\u5c31\u53ef\u4ee5\u53cd\u5e94\uff0c\u4e0d\u9700\u8981PCR\u4eea\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u4f46\u4f20\u7edfRCA\u6162\uff0c\u805a\u5408\u9176\u9700\u8981\u628a\u6574\u5708\u6a21\u677f\u90fd\u5408\u6210\u5b8c\uff0c\u624d\u80fd\u8fdb\u5165\u4e0b\u4e00\u8f6e\u6269\u589e\uff0c\u6548\u7387\u662f\u7ebf\u6027\u7684\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u4e2d\u539f\u5927\u5b66\u7814\u7a76\u56e2\u961f<strong>\u5728\u73af\u72b6\u6a21\u677f\u4e0a\u8bbe\u8ba1\u4e86\u591a\u4e2aNb.BbvCI\u5207\u523b\u4f4d\u70b9<\/strong>\u3002\u4ed6\u4eec\u505a\u4e86CT-S1\u3001CT-S2\u3001CT-S3\u4e09\u79cd\u6a21\u677f\uff0c\u5206\u522b\u5e261\u30012\u30013\u4e2a\u5207\u523b\u4f4d\u70b9\u3002phi29\u53ea\u9700\u8981\u5408\u6210\u73af\u72b6\u6a21\u677f\u7684\u4e09\u5206\u4e4b\u4e00\uff0cNb.BbvCI\u5c31\u80fd\u628a\u4ea7\u7269\u5207\u4e0b\u6765\uff0c\u5207\u4e0b\u7684\u77ed\u7247\u6bb5\u76f4\u63a5\u5f53\u4e0b\u4e00\u8f6e\u5f15\u7269\uff0c\u89e6\u53d1\u6307\u6570\u7ea7\u6269\u589e\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u8fd9\u4e2a\u8bbe\u8ba1\u5c06\u539f\u6765\u89811-2\u5c0f\u65f6\u7684\u6269\u589e\u8fc7\u7a0b\uff0c\u538b\u52306\u5206\u949f\u4ee5\u5185\u3002<\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<h2 style=\"color:#1f3a5f;margin:24px 0 12px;\">\u68c0\u6d4b\u6d41\u7a0b<\/h2>\n<p style=\"margin:12px 0;line-height:1.9;\"><strong>\u7b2c\u4e00\u6b65\uff0c\u6269\u589e<\/strong>\uff1a\u73af\u72b6\u6a21\u677f\uff08\u542bmiR-141\u8bc6\u522b\u533a+\u5207\u523b\u4f4d\u70b9\uff09\u9047\u5230miR-141\u624d\u5f00\u59cb\u542f\u52a8\uff0cphi29\u805a\u5408\u9176+\u652f\u6491DNA+Nb.BbvCI\u7ec4\u6210\u53cd\u5e94\u4f53\u7cfb\uff0c30\u00b0C\u6052\u6e296\u5206\u949f\uff0c\u6307\u6570\u7ea7\u6269\u589e\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\"><strong>\u7b2c\u4e8c\u6b65\uff0c\u8bd5\u7eb8\u6761\u53cd\u5e94<\/strong>\uff1a\u6269\u589e\u4ea7\u7269\u548c\u4e24\u4e2a\u63a2\u9488\u6742\u4ea4\uff0cbiotin\u63a2\u9488\uff08\u52fe\u4f4f\u94fe\u9709\u4eb2\u548c\u7d20\u4fee\u9970\u7684\u91d1\u7eb3\u7c73\u9897\u7c92AuNP\uff09\u548cFITC\u63a2\u9488\uff08\u88ab\u6d4b\u8bd5\u7ebf\u4e0a\u7684FITC\u6297\u4f53\u6355\u83b7\uff09\uff0c\u6d4b\u8bd5\u7ebf\u51fa\u7ea2\u8272\u5373\u4e3a\u9633\u6027\u53cd\u5e94\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u6ca1\u9776\u6807\u65f6\uff0c\u6e38\u79bbAuNP\u7ee7\u7eed\u5f80\u524d\u5c42\u6790\uff0c\u88ab\u8d28\u63a7\u7ebf\u7684\u94fe\u9709\u4eb2\u548c\u7d20\u6297\u4f53\u6293\u4f4f\uff0c\u53ea\u6709C\u7ebf\u7ea2\uff0c\u53cd\u5e94\u7ed3\u679c\u4e3a\u9634\u6027\u3002<\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<h2 style=\"color:#1f3a5f;margin:24px 0 12px;\">4 aM \uff1f<\/h2>\n<p style=\"margin:12px 0;line-height:1.9;\">\u4f18\u5316\u540e\u53c2\u6570\uff1a\u73af\u72b6\u6a21\u677f0.8 \u03bcM\u3001phi29\u805a\u5408\u917612 U\u3001Nb.BbvCI 15 U\u300130\u00b0C\u53cd\u5e946\u5206\u949f\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u8367\u5149\u6a21\u5f0f\uff1a\u9776\u6807\u4ece10 aM\u52301 nM\u4fe1\u53f7\u9012\u589e\uff0c100 nM\u9971\u548c\uff0c8\u5206\u949f\u5185\u5b8c\u6210\uff1b10 aM\u20131 nM\u7ebf\u6027\uff0c\u56de\u5f52\u65b9\u7a0by = 0.29 log C \u2212 6.64\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u8bd5\u7eb8\u6761\u6a21\u5f0f\uff1a\u6d4b\u8bd5\u7ebf\u989c\u8272\u5f3a\u5ea6\u5bf9\u9776\u6807\u6d53\u5ea6\u5bf9\u6570\u7ebf\u6027\uff0810 aM\u2013100 nM\uff0cR\u00b2 = 0.9908\uff09\uff0c\u63093\u500d\u6807\u51c6\u5dee\u7b97\uff0c<strong>LOD = 4 aM<\/strong>\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">\u7ebf\u6027\u8303\u56f4\u6db5\u76d610\u4e2a\u6570\u91cf\u7ea7\uff01<\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<h2 style=\"color:#1f3a5f;margin:24px 0 12px;\">\u5c3f\u6db2\u4e5f\u80fd\u4f5c\u4e3a\u6837\u672c<\/h2>\n<p style=\"margin:12px 0;line-height:1.9;\">\u5728\u5c3f\u6db2\u57fa\u8d28\u4e2d\uff0cLOD\u4e3a 8 aM\uff08\u7565\u9ad8\u4e8e\u7eaf\u6c34\u73af\u5883\u76844 aM\uff09\uff0c\u7ebf\u6027\u4e3a10 aM\u2013100 nM\uff0cR\u00b2 = 0.99\u3002<\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">16\u4efd\u52a0\u6807\u5c3f\u6db2\u6837\u672c\uff0c\u56de\u6536\u738799.69%\u2013102.32%\uff0cRSD\u4f4e\u4e8e5%\u3002\u7279\u5f02\u6027\u65b9\u9762\uff1amiR-21\u3001miR-155\u3001miR-92\u3001Let-7a\u56db\u4e2a\u5e72\u6270miRNA\u5168\u90e8\u65e0\u4fe1\u53f7\uff0c\u53ea\u6709miR-141\uff08\u548c\u542bmiR-141\u7684\u6df7\u5408\u6837\uff09\u51fa\u6761\u5e26\u3002<\/p>\n<hr style=\"border:none;border-top:1px solid #e3e8ef;margin:24px 0;\">\n<p style=\"margin:12px 0;line-height:1.9;\"><strong>\u4f60\u4eec\u62ff\u4ec0\u4e48\u8ddf\u6211\u73a9\u513f\uff01<\/strong><\/p>\n<p style=\"margin:12px 0;line-height:1.9;\">Teng Y-J, Huang Y-F, Wu S-R, et al. *Talanta*. 2026;305:129642. DOI: 10.1016\/j.talanta.2026.129642<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>10\u5206\u949f\uff0cLOD 4\u00d710\u207b\u00b3 fM\uff08=4 aM\uff09\uff0c\u65e0\u9700\u4eea\u5668\uff0c\u8089\u773c\u8bfb\u6570\u3002 4 aM\u662f\u4e2a\u4ec0\u4e48\u6982\u5ff5\uff1f \u53f0\u6e7e\u4e2d\u539f\u5927\u5b66 [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[7],"tags":[116,118,120,119,104,117,121,122],"class_list":["post-202","post","type-post","status-publish","format-standard","hentry","category-7","tag-erca","tag-lfa","tag-mir-141","tag-mirna","tag-104","tag-117","tag-121","tag-122"],"yoast_head":"<!-- This site is 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